H2N2V1, Main, Exploration, bibRecord, 000226

A newly developed tetraplex real‐time RT‐PCR for simultaneous screening of influenza virus types A, B, C and D

Identifieur interne : 000226 ( Main/Exploration ); précédent : 000225; suivant : 000227

A newly developed tetraplex real‐time RT‐PCR for simultaneous screening of influenza virus types A, B, C and D

Auteurs : Dinah Henritzi ; Bernd Hoffmann ; Silke Wacheck ; Stefan Pesch ; Georg Herrler ; Martin Beer ; Timm C. Harder

Source :

Influenza and Other Respiratory Viruses [ 1750-2640 ] ; 2018.

RBID : PMC:6304318

Abstract

Background

Human‐ or avian‐to‐swine transmissions have founded several autonomously circulating influenza A virus (IAV) lineages in swine populations that cause economically important respiratory disease. Little is known on other human influenza virus types, like B (IBV) and C (ICV) in European swine, and of the recently detected novel animal influenza virus type D (IDV).

Objectives

Development of a cost‐effective diagnostic tool for large‐scale surveillance programmes targeting all four influenza virus types.

Methods

An influenza ABCD tetraplex real‐time RT‐PCR (RT‐qPCR) was developed in the frame of this study. A selection of reference virus strains and more than 4000 porcine samples from a passive IAV surveillance programme in European swine with acute respiratory disease were examined.

Results

Two IBV, a single IDV but no ICV infections were identified by tetraplex RT‐qPCR. IBV and IDV results were confirmed by conventional RT‐PCR and partial sequence analysis.

Conclusions

The tetraplex RT‐qPCR proved fit for purpose as a sensitive, specific and high‐throughput tool to study influenza virus transmission at the human‐animal interface. Complementing close‐meshed active virological and serological surveillance is required to better understand the true incidence and prevalence of influenza virus type B, C and D infections in swine.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6304318

DOI: 10.1111/irv.12613
PubMed: 30264926
PubMed Central: 6304318

Affiliations:

Le document en format XML

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<author><name sortKey="Henritzi, Dinah" sort="Henritzi, Dinah" uniqKey="Henritzi D" first="Dinah" last="Henritzi">Dinah Henritzi</name>
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<author><name sortKey="Hoffmann, Bernd" sort="Hoffmann, Bernd" uniqKey="Hoffmann B" first="Bernd" last="Hoffmann">Bernd Hoffmann</name>
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<front><div type="abstract" xml:lang="en"><sec id="irv12613-sec-0001"><title>Background</title>
<p>Human‐ or avian‐to‐swine transmissions have founded several autonomously circulating influenza A virus (<styled-content style="fixed-case">IAV</styled-content>
) lineages in swine populations that cause economically important respiratory disease. Little is known on other human influenza virus types, like B (<styled-content style="fixed-case">IBV</styled-content>
) and C (<styled-content style="fixed-case">ICV</styled-content>
) in European swine, and of the recently detected novel animal influenza virus type D (<styled-content style="fixed-case">IDV</styled-content>
).</p>
</sec>
<sec id="irv12613-sec-0002"><title>Objectives</title>
<p>Development of a cost‐effective diagnostic tool for large‐scale surveillance programmes targeting all four influenza virus types.</p>
</sec>
<sec id="irv12613-sec-0003"><title>Methods</title>
<p>An influenza <styled-content style="fixed-case">ABCD</styled-content>
 tetraplex real‐time <styled-content style="fixed-case">RT</styled-content>
‐<styled-content style="fixed-case">PCR</styled-content>
 (<styled-content style="fixed-case">RT</styled-content>
‐<styled-content style="fixed-case">qPCR</styled-content>
) was developed in the frame of this study. A selection of reference virus strains and more than 4000 porcine samples from a passive <styled-content style="fixed-case">IAV</styled-content>
 surveillance programme in European swine with acute respiratory disease were examined.</p>
</sec>
<sec id="irv12613-sec-0004"><title>Results</title>
<p>Two <styled-content style="fixed-case">IBV</styled-content>
, a single <styled-content style="fixed-case">IDV</styled-content>
 but no <styled-content style="fixed-case">ICV</styled-content>
 infections were identified by tetraplex <styled-content style="fixed-case">RT</styled-content>
‐<styled-content style="fixed-case">qPCR</styled-content>
. <styled-content style="fixed-case">IBV</styled-content>
 and <styled-content style="fixed-case">IDV</styled-content>
 results were confirmed by conventional <styled-content style="fixed-case">RT</styled-content>
‐<styled-content style="fixed-case">PCR</styled-content>
 and partial sequence analysis.</p>
</sec>
<sec id="irv12613-sec-0005"><title>Conclusions</title>
<p>The tetraplex <styled-content style="fixed-case">RT</styled-content>
‐<styled-content style="fixed-case">qPCR</styled-content>
 proved fit for purpose as a sensitive, specific and high‐throughput tool to study influenza virus transmission at the human‐animal interface. Complementing close‐meshed active virological and serological surveillance is required to better understand the true incidence and prevalence of influenza virus type B, C and D infections in swine.</p>
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A newly developed tetraplex real‐time RT‐PCR for simultaneous screening of influenza virus types A, B, C and D

A newly developed tetraplex real‐time RT‐PCR for simultaneous screening of influenza virus types A, B, C and D

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Abstract

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